Team:NCKU Tainan/Protocols 2019.igem.org
1. Excise band with scalpel and transfer to a new eppendorf tube. 2. Weigh the gel slice in a tube by measuring the weight difference of an empty eppendorf and the eppendorf with gel slice in the tube. 3. Add 3 volumes of binding buffer to 1 volume of gel 100 mg = 100 μl. 4. Incubate at 60°C for 2 10 mins or until the gelpletely





































