Grinding of leaves for extraction of nucleic acids
Either the mortar and pestle can be pre chilled and the grinding performed dry on frozen leaves, or the leaves can be submersed in liquid nitrogen for the grinding. Cryogenic grinding is a very effective technique for taking hard substances, like plant and animal tissues, and turning them into dust. The tough carbohydrates of plant tissuese very fragile at 196°C and easily shatter. The





































